SLE患者血中调节性T细胞及TGF-β_1的表达

Peripheral Blood Expression of T Regulatory Cells,TGF-β_1 and Anti-ds DNA Antibody in Patients with SLE

目的:检测系统性红斑狼疮(SLE)轻型与重型患者中调节性T细胞、TGF-β1和抗ds-DNA抗体的表达水平,并与正常健康人对比,分析它们之间的差异。方法:应用放射免疫分析和ELISA检测血清抗ds-DNA和TGF-β1的表达水平,采用流式细胞仪检测调节性T细胞的表达水平。结果:狼疮重型组中调节性T细胞显著低于狼疮轻型组和健康对照组(P<0.05);重型组抗ds-DNA抗体显著高于轻型组和健康对照组(P<0.05);重型与轻型的TGF-β1的表达水平差异无统计学意义,但重型组和轻型组的表达水平都比健康对照组明显降低。结论:调节性T细胞的表达数量可作为狼疮活动的指标,而血清TGF-β1的表达水平与其活动性无关。

Objective To study the changes of peripheral blood expression of T regulatory cells, TGF-β1 and anti-dsDNA antibody in patients with SLE. Methods Peripheral blood T regulatory cell proportion （with flow-cytometry） and serum anti-dsDNA antibody （with RIA）, TGF-β1 （with ELISA） expressions were examined in ① 94 SLE patients in active stage ② 60 SLE patients in stable stage and ③ 60 controls. Results The T regulatory proportion in SLE patients in active stage was significantly lower than that in SLE patients in stable stage and controls （P〈0.05）, while the anti-dsDNA antibody expression was significantly higher （P also 〈0.05）. The TGF-β1 expressions in the active and stable SLE patients were not significantly different, but they were significantly lower than that in controls. Conclusion Peripheral blood T regulatory cell proportion decreased during active stage in SLE patients, but TGF-β1 expression was not related to the activity of the disease.