重症乙型肝炎患者中西医结合治疗前后血清IL-12、TGF-β、CTGF和PDGF水平变化的临床意义

Clinical Significance of Changes of Serum IL-12,TGF-β,CTGF and PDGF Levels After Treatment with Integrated Traditional and Western Medicine in Patients with Chronic Severe Hepatitis B

目的:探讨重症乙型肝炎患者中西医结合治疗前后血清相关细胞因子水平的变化及临床意义。方法:将50例重症乙型肝炎患者分为治疗前和治疗后组进行研究;血清TGF-β含量采用放射免疫分析;结缔组织生长因子(CTGF)、血小板衍生长因子(PDGF)和IL-12测定均采用酶联免疫吸附法。结果:IL-12水平治疗前组显著高于对照组(P<0.01),治疗后水平下降不显著,仍显著高于对照组(P<0.01)。TGF-β含量治疗前组显著高于对照组(P<0.01);治疗后水平下降显著(P<0.05),但与对照组比较仍呈显著高水平(P<0.05)。CTGF浓度治疗前组显著高于对照组(P<0.01),治疗后下降显著(P<0.05),但仍显著高于对照组(P<0.05)。PDGF水平治疗前组显著高于对照组(P<0.01),治疗后显著下降(P<0.05),但仍非常显著地高于对照组(P<0.01)。结论:重症乙型肝炎患者血清TGF-β、CTGF、IL-12及PDGF四项血清指标的测定对于研究患者的病情进展及预后评估提供了有价值的实验依据。

Objective To investigate the relationship between progress of disease process and changes of serum IL-12, TGF-β, CTGF and PDGF levels in patients with chronic severe hepatitis B. Methods Serum TGF-β （with RIA） and IL-12, connective tissue growth factor （CTGF） platalet-derived growth factor （PDGF） （all with ELISA） levels were determined both before and after integrated traditional and western medicine treatment in 50 patients with chronic severe hepatitis β as well as once in 50 controls. Results Before treatment the serum levels of IL-12 were significantly higher in patients with chronic severe hepatitis B than those in the controls （P〈0.01）, while after treatment, the serum levels of IL-12 were only slightly decreased and remained significantly higher than those in the controls （P〈0.01）. Before treatment the serum levels of TGF-β, CTGF and PDGF were all significantly higher than those in controls （P〈0.01）. After treatment, the levels all dropped significantly （vs before treatment, P〈0.05）, but still remained significantly higher than those in controls （TGF-β, P〈0.05, CTGF and PDGF, P〈0.01）. Conclusion Detection of changes of IL-12, TGF-β, CTGF and PDGF levels after treatment in patients with chronic severe hepatitis B provided a valuable laboratory basis for studing the progress of disease process.