自然增龄雄性大鼠BMD、BALP、TRACP5b的变化研究

The changes of BMD ＆ BALP ＆ TRACP5b in ageing male rats

目的观察自然增龄情况下雄性SD大鼠骨密度（bone mineral density，BMD）、骨碱性磷酸酶（bonealk aline phosphatase，BALP）、抗酒石酸酸性磷酸酶5b（tartrate—resistant acid phosphatase 5b，TRACP5b）的变化情况。方法选取自然进程中2m（10只）、3m（11只）、10m（10只）、11m（15只）共4个月龄段的雄性SD大鼠，检测大鼠的腰椎和股骨的BMD水平，血清BALP和TRACP5b水平。结果雄性大鼠在2月龄到11月龄过程中，股骨和腰椎BMD显著升高（P=0．000），BALP保持平稳（P=0.975），TRACP5b显著下降（P=0．000）.结论自然增龄使雄性大鼠的骨密度明显升高，这可能主要与TRACP5b水平的降低变化有关，而与BALP的关系不明显。

Objective To investigate the changes of BMD, BALP, TRACPSb in male rats during natural increasin g age. Methods SD rats with the age of 2-month （n=10）, 3-month （n=11）, 10-month （11=10）, 11-month （n = 15 rats ） were studied in the experiment. Bone mineral density （BMD） of rat lumbar spine and femur was detected, while bone alkaline phosphatase （BALP） and tartrate-resistant acid phosphatase 5b （TRACPSb） in rat serum were assessed, respectively. Results During natural increasing age from 2 months to 11 months, the levels of BMD of the rat femur and lumbar spine increased significantly （P = 0.000）, the levels of the serum BALP remained stable （P = 0.975）, and the levels of serum TRACPSb decreased significantly （P = 0.000）. Conclusion Natural aging significantly increases rat bone mineral density, which may mainly involve the changes in decreasing of rat serum TRACP5b rather than rat serum BALP.