硫辛酸对甲醛致人HepG2细胞DNA损伤的保护作用

Protecting Role of Lipoic Acid on DNA Damage Induced by Formaldehyde in Human HepG2 Cells

为探讨硫辛酸对甲醛诱发人HepG2细胞DNA损伤的保护作用,体外培养的HepG2细胞经甲醛(0.25,2.5,25μmol/L)单独处理或甲醛与硫辛酸((5+20),(5+40)μmol/L)同时处理2 h后,分别运用彗星试验(comet assay)结合彗星图像分析软件(CASP)分析细胞尾部DNA百分率(tail DNA%)变化.结果显示:不同浓度甲醛处理后HepG2细胞尾部DNA百分率以浓度依赖性方式显著升高(P<0.01);但甲醛与硫辛酸同时处理后HepG2细胞尾部DNA百分率明显降低并与硫辛酸浓度呈依赖关系,与甲醛单独处理组(5μmol/L)相比差异显著(P<0.05或P<0.01).此结果说明硫辛酸对甲醛诱发的人HepG2细胞DNA损伤有保护作用.

To investigate the protecting role of lipoic acid on DNA damage induced by formaldehyde in human HepG2 cells,cultured HepG2 cells were treated with formaldehyde（0.25,2.5,25 μmol/L） alone or treated with formaldehyde and lipoic acid together（（5＋20）,（5＋40） μmol/L） for 2 h.After the treatment,the tail DNA % of HepG2 cells was analyzed using the comet assay combined with the comet image analyzing software（CASP）.The results have showed that when treated with different concentrations of formaldehyde alone, the tail DNA ~ of HepG2 cells has significantly increased with the concentration-effect dependent manner （P〈0.01）. However, when treated with formaldehyde and lipoic acid together, the tail DNA ~ of HepG2 cells has obviously decreased with the lipoic aci＆dependent mode, and has distinct difference comparing to the formaldehyde alone（5μmol/L）-treated cells （P〈0.05 or P〈0.01）. The paper has concluded that lipoic acid has the role of protecting the formaldehyde induced DNA damage in HepG2 cells.