摘要
目的:采用高效液相色谱法(HPLC)测定避孕套中苯佐卡因(benzocaine)的含量。材料与方法:采用Polaris C18-A(5μm 250×4.6mm)色谱柱,用乙腈-水60:40作为流动相,检测波长为265nm,流速:1.0ml*min-1,进样量10μl。结果:苯佐卡因在98.5μg/ml^492.5μg/ml范围内呈良好的线性关系(相关系数R2=0.9998),精密度(RSD)0.7%,平均回收率为102.4%。结论:该方法操作简便可行,适用于含苯佐卡因避孕套中苯佐卡因含量的质量控制。
Objective： To establish a HPLC method for determination of benzocaine in condom.Methods： A Polaris C18-A column（5μm 250×4.6mm） was used. The mobile phase was composed of acetonitrile-water （60：40）and its flow rate was 1.0ml/min. The detection wavelength was at 265nm.Results： The calibration curve was linear in the range of 98.5μg/ml to 492.5μg/ml（R2=0.9998）.The average recovery was 102.4%,RSD=0.7%.Conclusion： This method is simple and accurate.It can be applied for determination of benzocaine in condom.
出处
《中国医疗器械信息》
2010年第12期37-38,共2页
China Medical Device Information
