土茯苓抗人巨细胞病毒的体外实验研究

Experimental study on the anti-cytomegalovirus effect of smilax glabra in vitro

目的:探讨土茯苓体外抗HCMV的效果和安全性。方法:用细胞病变法和MTT法,检测土茯苓体外抗人巨细胞病毒的最大无毒浓度、最小有效浓度和治疗指数,并与更昔洛韦、复方中药金叶败毒进行比较。结果:更昔洛韦、金叶败毒、土茯苓的最大无毒浓度分别为10μg/ml、3000μg/ml、30000μg/ml,最小有效浓度分别为10μg/ml、300μg/ml、300μg/ml,治疗指数分别为1、10、100。结论:土茯苓细胞毒性略低于金叶败毒,大大低于更昔洛韦;抗HCMV效果与金叶败毒相同,略低于更昔洛韦;治疗指数最高,有可能成为治疗HCMV活动性感染的药物。

Objective：To explore the anti-cytomegalovirus efficacy and safety of smilax glabra in vitro.Methods：Cytopathic assay and MTT method were used to detect the maximum atoxic concentration,minimum effective concentration and therapeutic index of anti-cytomegalovirus effect of smilax glabra in vitro,then the results were compared with ganciclovir and compound Jinyebaidu.Results：The maximum atoxic concentrations of ganciclovir,compound Jinyebaidu and smilax glabra were 10 μg/ml,3 000μg/ml and 30 000μg/ml,respectively;the minimum effective concentrations of ganciclovir,compound Jinyebaidu and smilax glabra were 10 μg/ml,300 μg/ml and 300 μg/ml,respectively;the therapeutic indexes of ganciclovir,compound Jinyebaidu and smilax glabra were 1,10 and 100,respectively.Conclusion：The cytotoxicity of smilax glabra is lower than that of compound Jinyebaidu,which is significantly lower than that of ganciclovir;the anti-cytomegalovirus effect of smilax glabra is similar to that of compound Jinyebaidu,which is lower than that of ganciclovir;the therapeutic index of smilax glabra is the highest,which may become to be a new drug for treatment of active infection of cytomegalovirus.