GnRHα超促排卵对围着床期小鼠子宫内膜LPAR3表达的影响

Effect of controlled ovarian hyperstimulation by GnRHα on the expression of LPAR3 in endometrium of mice during peri-implantation period

目的：研究GnRHα控制性超促排卵对围着床期小鼠子宫内膜溶血磷脂酸受体3（LPAR3）mRNA和蛋白表达的影响。方法：采用RT-PCR、Western blot方法检测自然受孕组（NC）和超促排卵组（COH）小鼠性交后第3~6天（3~6d.p.c）子宫内膜LPAR3mRNA和蛋白表达的表达。应用免疫化学发光法测定血清雌、孕激素浓度。结果：COH组3d.p.cLPAR3mRNA和蛋白的表达最强,NC组4d.p.c表达最强。其余相应各时间点两组间表达量差异无统计学意义;雌、孕激素在两组均随着妊娠天数增加而升高。相应同期各时间点COH组高于NC组（P〈0.05）。结论：COH可能通过改变与子宫内膜容受性相关的LPAR3的时序变化影响子宫内膜容受性,从而影响胚胎着床。

Objective：To investigate the effect of controlled ovarian hyperstimulation by GnRHα on the expressions of lysophosphatidic acid receptor 3（LPAR3）mRNA and protein in endometrium of mice during peri-implantation period.Methods：PT-PCR and Western blot were used to detect the expression levels of LPAR3 mRNA and protein in endometrium of mice at 3~6 days after sexual intercourse in natural conception group and controlled ovarian hyperstimulation group;the serum levels of estrogen and progesterone were detected by immunochemistry luminescence method.Results：The expression levels of LPAR3 mRNA and protein on the third day after sexual intercourse in controlled ovarian hyperstimulation group were the highest;the expression levels of LPAR3 mRNA and protein on the fourth day after sexual intercourse in natural conception group were the highest;there was no significant difference in the other days between the two groups;the serum levels of estrogen and progesterone in the two groups increased with the progress of pregnancy;at the same time points,the serum levels of estrogen and progesterone in controlled ovarian hyperstimulation group were significantly higher than those in natural conception group（P0.05）.Conclusion：Controlled ovarian hyperstimulation may affect endometrial receptivity by changing LPAR3,which may affect implantation.