戊二醛鞣制VBJVC流体动力学的体外实验研究

Hydrodynamic experimental study on VBJVC tanned by GA in vitro

目的体外测试戊二醛(GA)鞣制牛颈外静脉带瓣管道(VBJVC)流体动力学的相关特性。方法用自行制作的流体动力学测量仪,对20根经GA鞣制的VBJVC进行逆瓣膜及顺瓣膜灌流量测定,测量跨瓣压差及瓣膜管道逆行加压实验。并用光镜检查管道及瓣膜形态结构改变。结果 (1)逆/顺行灌流量百分比(P)。分为3组:A组(P<20%)11根;B组(20%≤P≤50%)5根;C组(P>50%)4根。(2)跨瓣膜压差很小,为0～1 mm Hg。(3)逆行加压实验,光镜检查未发现瓣膜及管壁损伤,上皮细胞、纤维细胞、胶原纤维及弹力纤维均完整无损。(4)瓣膜抗反流功能与瓣叶高度和瓣窦高度之比呈正相关(P<0.01)。结论经GA鞣制的VBJVC抗反流能力与瓣叶高度和瓣窦高度之比呈正相关。瓣膜开放功能好。逆行加压试验显示瓣膜及管道结构无损伤。

Objective To test the hydrodynamic characteristics of Valved bovine jugular vein conduit（VBJVC）tanned by glutaraldehyde（GA）outside the body.Methods Both antidromic and antegrade perfusion flow of 20 VBJVC tanned by GA were measured with a hydrodynamic admeasuring apparatus made by ourselves.The transvalvular pressure gradient of these VBJVC was measured and the antidromic pressurization test of the valved conduit.Finally,light microscopy to examine the morphological structure change of the valved conduit.Results（1）The percentage of antidromic and antegrade perfusion flow（P）.we divide 3 groups,Group A：11 VBJVCs,P〈20%;Group B：5 VBJVCs,20%≤P≤50%;Group C：4 VBJVCs,P50%.（2）The transvalvular pressure gradient ranging from 0 to 1 mm Hg is small.（3）The pressurization test of antidromic perfusion：light microscopy find out the epithelial cells,fibroblasts,collagen fibers,and elastic fibers all remained intact.（4）The anti-reflux function of valves had a positive correlation with the ratio of the height of the valve leaflets to the height of valve sinus（P〈0.01）.Conclusion VBJVCs tanned by GA had a positive correlation with the ratio of the height of the valve leaflets to the height of valve sinus.VBJVC valves performed good opening function When the pressurization test of antidromic perfusion was carried out,while no damage was found in valves and conduits.