高效液相色谱法测定胃灵胶囊中芍药苷含量

Determination of Paeoniflorin in Weiling Capsules by HPLC

目的建立测定胃灵胶囊中芍药苷含量的高效液相色谱法。方法采用Hypersil ODS色谱柱(150 mm×4.6 mm,10μm),流动相为乙腈-0.1%磷酸溶液(15∶85),检测波长为229 nm,流速1 mL/min,柱温30℃。结果芍药苷质量浓度在5.1～25.5μg/mL范围内与峰面积线性关系良好,r=0.999 9(n=5);平均回收率为97.30%,RSD=3.02%(n=9)。结论高效液相色谱法重现性好、简便易行,可用于胃灵胶囊的质量控制。

Objective To establish an HPLC method for the determination of paeoniflorin in Weiling Capsules.Methods To use the Hypersil ODS column（150 mm×4.6 mm,10 μm）,the mobile phase was acetonitrile-0.1%phosphoric acid（15 ∶85）,the detection wavelength was 229 nm,the flow rate was 1 mL/min and the column temperature was 30 ℃.Results The linear range of paeoniflorin was 5.1-25.5 μg/mL,r=0.999 9.The average recovery rate was 97.30%,RSD was 3.02%（n=9）.Conclusion The method was simple and accurate,which can be used for the quality control of Weiling Capsules.