吕梁地区脑梗死患者血浆ICAM-1及K469E基因多态性研究

Levels of Plasma Intercellular Adhesion Molecule-1 and K469E Gene Polymorphism in Patients with Cerebral Infarction in Luliang Region

目的检测吕梁地区脑梗死患者血浆细胞间粘附分子-1(ICAM-1)水平及其基因K469E多态性,探讨其与脑梗死的关系。方法脑梗死组115例,对照组99例,采用ELISA法检测ICAM-1血浆水平,PCR-RFLP法分析K469E基因多态性。结果脑梗死组ICAM-1血浆水平显著高于对照组(t=7.472,P<0.05);其中大面积梗死组血浆ICAM-1水平显著高于中等面积和腔隙性梗死组(P<0.05),中等面积和腔隙性梗死组相比较差异无统计学意义(P>0.05)。基因K469E基因型频率和等位基因频率在脑梗死组和对照组比较差异有统计学意义(P<0.05),E等位基因携带者患脑梗死的风险是K等位基因的1.951倍。结论 ICAM-1血浆水平及其基因K469E多态性与脑梗死的发病具有相关性,且血浆ICAM-1水平与梗死灶面积相关,E等位基因可能是吕梁地区脑梗死发病的遗传易感基因。

Objective To detect the plasma levels＇of ICAM-I and its K469E gene polymorphisms in patients with cerebral infarction in Luliang region. Methods 115 cerebral infarction patients and 99 normal controls were enrolled in this study. ELISA was used to measure plasma ICAM-1 levels,and PCR-RFLP was inducted to assay K469E polymorphism. Results ICAM-1 plasma levels of cerebral infarction group were significantly higher（ t = 7. 472, P 〈 0.05 ） than that of the control group;the plasma levels of ICAM-1 in patients with large area cerebral infarction was significantly higher than the patients with the middle and small area （ P 〈 0.05 ） , while there was no significant difference between middle area group and small area infarction （ P 〉 0.05 ）. There was a significant difference（ P 〈 0.05 ） about Gene K469E genotype and allele frequencies between the cerebral infarction group and control group. The risk of suffering from cerebral infarction in E allele was 1. 951 times than K allele. Conclusion The plas- ma level of ICAM-1 was correlated with cerebral infarction, and was correlated with infarct area. ICAM-1 gene K469E polymorphism and cerebral infarction is relevant. E allele may be a genetic susceptibility gene of cerebral infarction in Luliang area.