摘要
目的 探讨不同浓度的甲基转移酶抑制剂5-脱氧杂氮胞苷（5-Aza-CdR）对上皮性卵巢癌细胞系ES2、SKOV3细胞生长和体外侵袭力的影响.方法 应用甲基化特异性PCR检测上皮性卵巢癌细胞系ES2及SKOV3 E-cad基因启动子区5＇CpG岛甲基化状况.观察ES2和SKOV3用5-Aza-CdR处理前后细胞的形态变化.用噻唑蓝（MTT）法检测细胞生长情况并绘制生长曲线.用Transwell 小室法检测药物处理前后细胞侵袭力的变化.结果 经不同浓度5-Aza-CdR处理后SKOV3细胞的穿膜细胞数发生了明显变化,分别为对照组（97.6±2.7）个,5-Aza-CdR 0.1μmol/L组（88.2±2.1）个,5-Aza-CdR 1 μmol/L组（60.5±2.2）个,5-Aza-CdR 10 μmol/L组（36.2±3.0）个,随着药物浓度的增高穿膜细胞数逐渐下降,在10 μmol/L浓度时穿膜细胞数最少.结论 甲基转移酶抑制剂5-Aza-CdR能够使卵巢癌细胞的恶性生物学行为发生部分逆转,其在卵巢癌治疗方面的价值值得进一步探讨.
Objective To observe the effects of different concentrations of 5 - aza cdr （ methyl transferase inhibitor） on epithelial ovarian cancer cell line ES2, SKOV3 growth inhibition and invasive capacity. Methods Methylation-specific polymersse chain reaction was applied to detect epithelial ovarian cancer cell line ES2, 3AO and SKOV3 E-cad gene promoteR5＇CpG island methylation status. The morphological change of ES2 and SKOV3 before and after treatment with 5- Aza CdR was observed. Thiazolyl blue （MTT） method was used to detect cell growth and the growth curve. The change of cell invasive capacity was detected by transwell chamber method before and after treatment. Results After being treated by different concentrations of 5-Aza-CdR, invasive cell numbers in SKOV3 changed dramatically. Invasive cell numbers of the control group, 5-Aza 0. 1 μmol/L group, 5-Aza 1 μ mol/L group and 5-Aza 10 μmol/L group were （97.56 ±2.67）, （88.23 ±2.12）, （60.46 ±2.25） ,（36.21 ±2.98）, respectively. With the increase of drug concentration,the number of penetrating cells decreased. Conclusion Methyl transferase inhibitoR5-Aza-CdR can partially reverse the malignant behavior of ovarian cancer cells.
出处
《中国医药》
2011年第1期106-108,共3页
China Medicine
