不同采收期凤丹皮中3种药效成分动态变化研究

Research on Dynamic Changes of Three Effective Components of Moutan Cortex at Different Harvesting Periods in Anhui Nanling

目的通过研究不同采收期安徽南陵凤丹皮3种药效成分的动态变化,确立凤丹皮的最佳采收期,为牡丹皮GAP生产提供依据。方法采用高效液相色谱法,以甲醇-乙腈(1:1)-0.5‰甲酸为流动相进行梯度洗脱,在265nm波长处,同时测定不同生长发育期凤丹皮中没食子酸、丹皮酚、芍药苷3种化学成分的含量。结果凤丹皮中3种药效成分的含量不同时期存在差异,但均以9月份为最高。结论9月份为安徽凤丹皮的最佳采收时期;该法简便、精密度高、重复性好,为有效地控制牡丹皮的内在质量提供了科学依据。

Objective By studying the dynamic changes of three effective components of moutan cortex at different harvesting periods in Anhui Nanling, to establish the best harvesting time and provide basis of GAP. Methods HPLC was used, with the mobile phase of methanol-acetonitrile （1 ： 1）-0.5%0 formic acid, to detect the gallic acid, paeonol and paeoniflorin in 265 nm wavelength at the same time. Results Three kinds of chemical components contents of moutan cortex in different harvesting periods were different, but all was the highest in september. Conclution September is the best harvesting time for moutan cortex. This method is simple, precise and repeatable, and provide the scientific reference to effectively control the internal quality of moutan cortex.