内蒙古盐生肉苁蓉中松果菊苷和麦角甾苷的含量测定

Determination of echinacoside and acteoside in herbs of Cistanche salsa(C.A.Mey.) G.Beck of Inner Mongolia

目的:采用高效液相色谱法,测定盐生肉苁蓉中有效成分松果菊苷和麦角甾苷的含量。方法:Agilent Eclipse XDB-C18色谱柱(4.6mm×250mm)。流动相:乙腈-甲醇-1%醋酸溶液(9:14:77)。流速:1.0mL/min,柱温为室温,检测波长334nm。结果:松果菊苷在0.0258mg-0.774mg呈良好的线性关系,回归方程为Y=2141.5X-35.72,r=0.9999;麦角甾苷在0.030mg-0.750mg呈良好的线性关系,回归方程为Y=4065.6X+159.35,r=0.9999。松果菊苷、麦角甾苷平均加样回收率分别为102.2%、103.9%。结论:本法测定简便、快速、灵敏、重复性好,方法可行。

Objective： To analyse the echinacoside and acteoside content of Cistanche salsa determination by RP-HPLC method.Method： An Agillent Eclipse XDB-C18（4.6mm×250mm）.Column was used and a mixture of acetonitrile-methanol-1% acetic acid（9：14：77）.Was used as the mobile phase at a flow rate of 1.0ml·min-1.The column temperature was room and the UVdetection wavelength was 334 nm.Results： The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.0258mg-0.774mg（r=0.9999） and 0.030 mg-0.750mg（r=0.9999） respetively and the average recoveries of echinacoside（and acteoside） were102.2%,103.9%.Conclusion： The method is simple,rapid,accurate and feasible.