不同频率电针对实验性肥胖大鼠脂代谢的影响

Effect of different-frequency electroacupuncture on lipid metabolism in obesity rats

目的:通过观察不同频率的电针对实验性肥胖大鼠脂代谢的影响,探讨频率调节脂代谢异常的作用机制。方法:采用谷氨酸钠和高脂饮食诱导的下丘脑性肥胖大鼠模型,选取大鼠曲池、丰隆、三阴交、后三里、中脘和关元穴,采用2Hz、50Hz、100Hz频率针刺。测定大鼠体质量指数(Lee,s指数)、左肾周脂肪和大网膜脂肪湿重;用酶学终点法测血清三酰甘油(TG)、胆固醇(TC)的含量;用磷钨酸-镁沉淀法测血清高密度脂蛋白胆固醇(HDL-C)含量;用聚乙烯硫酸沉淀法测血清低密度脂蛋白胆固醇(LDL-C)含量。结果:与模型组比较,电针3组大鼠Lee,s指数、左肾周脂肪和大网膜脂肪湿重、血清TG、TC和LDL-C含量均显著降低(P<0.05或P<0.01),血清HDL-C含量显著升高(P<0.05)。结论:2Hz、50Hz、100Hz电针频率降低大鼠Lee,s指数、调整脂代谢的作用有依次递减的趋势,50Hz电针频率似在降低脂肪重量上较2Hz和100Hz有优势。

Objective： To observe the effect of different-frequency electroacupuncture on lipid metabolism in obesity rats,and to analyze the regulation mechanism of frequency to the disorder of Lipid Metabolism.Methods： The hypothalamus obesity rat model was induced by sodium glutaminic acid and high lipoprotein diet,adopt 2Hz,50Hz and 100Hz frequency to acupuncture quchi,fenglong,sanyingjiao,housanli,zhongwan and guanyuan.To determine the rat＇s weight index（Lee＇s index）,the wet weight of left kidney cycle and big omentum;Bloodsamples were collected for assaying serumtriglyceride（TG）,total cholesterol（TC） with cholesterol oxidase phenol 4-aminoantipyrine peroxidase（CHOD-PAP） method,glycerol phosphate oxidase-p-aminophenazone（GPO-PAP）methods,high density lipid-cholesterol（HDL-C） with phosphotungstic acid-Mg2＋ method,low density lipid-cholesterol（LDL-C） with polyvingel sulfate（PVS） sedimentation method.Results： Compared with model group,Lee＇s index,the wet weight of left kidney cycle and big omentum,serum TG,TC and LDL-C contents of 3 electric needle groups decreased considerably（P0.05 or P0.01）,serum HDL-C content increased（P0.05）.Conclusion： There is a gradually decreased tendency of 2Hz,50Hz and 100Hz electric needle to reduces the Lee＇s indexand lipid metabolism,compared with 2Hz and 100Hz electric needles,there is a relatively advantage of 50Hz electric needle in reducing fat weight.