大鼠肠黏膜组织中谷氨酰胺测定方法的建立及应用

HPLC determination and application of glutamine content in rats intestines mucous membrane tissues

目的建立一种准确、简便、快速的高效液相色谱法,用于测定大鼠肠黏膜组织中谷氨酰胺的含量。方法以异硫氰酸苯酯作柱前衍生剂,反相C18柱为固定相,以50mmol/L醋酸钠缓冲液(pH6.05)∶乙腈=94∶6为流动相进行洗脱,在254nm紫外波长处测定谷氨酰胺的含量。结果在0.03～2.50mmol/L之间线性关系良好(r=0.9965),回收率为82.16%～115.22%。结论该方法简便、准确,适用于对肠黏膜组织中谷氨酰胺含量的测定分析。

Objective To establish one kind of accurate,simple and fast high performance liquid phase chroma- tography （HPLC） method to determine the glutamine content in rats intestines mucous membrane organization. Methods To take phenyl isothiocyanate （PITC） as the preco/umn derivatization agent,the reverse phase C18 column as the fixed phase and 50 mmol/L sodium acetate buffer solution （pH 6.05） ： acetonitrile （94 ： 6 ） as the mobile phase for carrying out elution. The glutamine content was determined at the ultraviolet wavelength of 254 nm. Results The good linearity was in the range of 0. 03-2. 5 mmol/L （r=0. 996 5）. The recovery rate was 82. 16%--115.22%. Conclusion This method is simple and accurate,which is suitable for the glutamine content determination in intestines mucous membrane tissues.