原发性肝癌患者可溶性Tie-2对血管新生的作用

Role of sTie-2 in angiogenesis of primary hepatic carcinoma

【目的】通过检测血清中sTie-2的表达,研究可溶性Tie-2与原发性肝癌血管新生的相关性,探讨可溶性Tie-2在原发性肝癌血管新生中的作用。【方法】采用夹心ELISA法检测32例原发性肝癌患者和15例肝硬化患者术前血清中可溶性Tie-2以及15例健康献血员血清中可溶性Tie-2的表达水平。采用免疫组化技术S-P法,检测肝癌组织、肝硬化组织和肝破裂之正常肝组织中Ang-2和Tie-2的表达。【结果】肝癌患者术前血清中sTie-2比肝硬化和健康鲜血员血清中sTie-2明显增高,而肝硬化与健康献血员血清中sTie-2差异无显著性。可溶性Tie-2在原发性肝癌患者血清中表达明显上调。【结论】可溶性Tie-2在原发性肝癌血管新生中起重要作用s。

【Objective】 Through sTie-2 is detected in the preoperative serums of primary hepatic carcinoma（PHC） and hepatic cirrhosis（HC） and serums of healthy blood donor.To study correlation soluble Tie-2（sTie-2） and PHC.To investigate the role of soluble Tie-2 in angiogenesis of PHC.【Methods】 Expression levels were detected in preoperative serums of HC and PHC were obtained from patients with hepatic cirrhosis or primary hepatic carcinoma by ELISA technique,and serums of healthy blood donor act as control group.Through streptavidin-biotin-peroxidase complex technique（S-P technique） of immunohistochemistry,Ang-2 and Tie-2 were detected in hepatoma tissues,tissues of HC and normal tissues.【Results】 The expression levels of sTie-2 in preoperative serum of primary hepatic carcinoma are higher than those of hepatic cirrhosis and healthy persons.The expression levels of sTie-2 between hepatic cirrhosis and healthy persons are no significance in statistics.Soluble Tie-2 were significantly up-regulated in preoperative serums of PHC compare to preoperative serums of PHC and serums of healthy blood donor.【Conclusion】 Soluble Tie-2 may play critical role in promoting tumor angiogenesis of PHC,the expression levels of sTie-2 in preoperative serum of PHC offer a new index for clinical screening and early diagnose of PHC.