超临界CO2流体用于萃取酸浆宿萼中类胡萝卜素的研究

Extraction of carotenoids from calyx of Physalis alkekengi var. francheti by supercritical CO_2

采用超临界CO2流体从酸浆宿萼中提取类胡萝卜素,探讨了萃取压力、温度、时间和原料粒度等萃取参数对提取量的影响。还利用UV定量地考察了所提取类胡萝卜素的耐光照稳定性、耐热稳定性和抗金属离子稳定性。结果表明,这些提取物耐热稳定,但对光很敏感。与金属离子的相互作用结果显示,Fe3+、Fe2+和Al3+对类胡萝卜素提取物的影响很显著,Cu2+、Zn2+和Ca2+等金属离子的影响相对较小,而Na+和K+基本没有影响。

The supercritical CO2 extraction has been examined for carotenoids extraction from Physalis alkekengi var.francheti calyx.The influences of the extraction pressure,temperature,time and particle size on the extraction yield were investigated.By means of UV spectrometry analysis,the stability of the extracted carotenoids was also investigated under several different conditions.The results showed that the obtained carotenoids were thermostable but very sensitive to sun-shining light.They were also strongly affected by some metal ion such as Fe^3＋ , Fe^2＋ and Al^3＋ ,relatively weakly influenced by Cu^2＋ ,Zn^2＋ and Ca^2＋ ,and nearly not affected by two earth ions of Na^＋ and K^＋.