红松仁蛋白氨基酸组成分析及营养评价

Study on content of amino acid in the pine seed protein and its nutritive evaluation

以红松仁为原料,利用氨基酸自动分析仪检测红松仁可溶性蛋白中的氨基酸组成,以氨基酸比值系数分和WHO/FAO氨基酸评分为评价标准,对红松仁蛋白质营养价值进行全面的评价。结果表明:红松仁蛋白中氨基酸种类齐全,谷氨酸含量最高,必需氨基酸的含量占氨基酸的总量为25%,属于优质植物蛋白。红松仁中水溶和盐溶蛋白占红松仁蛋白的67.7019%,水溶蛋白和醇溶蛋白的氨基酸组成及评分最为合理,是红松仁组成蛋白中较优的蛋白组分;按照氨基酸比值系数,红松仁组成蛋白中,水溶蛋白(82.9256)>碱溶蛋白(81.453)>醇溶蛋白(79.0838)>总蛋白(77.1625)>盐溶蛋白(46.3652)。总蛋白、水溶蛋白、盐溶蛋白的第一限制氨基酸为缬氨酸,碱溶蛋白和醇溶蛋白的第一限制性氨基酸为蛋氨酸和胱氨酸。

Using the score of ratio coefficient of amino acid and WHO/FAO reference model of essential amino acid （EAA）as an apprasial criterion,the nutritional value of pine seed protein were evaluated by ratio coefficient acid （AA）.The results showed that the AA in pine seed protein were rich in all kinds of AA,Glutamic acid was the most high in total amino acid,the EAA 25% of total AA,which was high-quality plant protein.The water-soluble protein and salt-soluble protein were 67.7019% in pine seed protein.The contents and scores of the water-soluble protein and salt-soluble protein were reasonable.Using the score of ratio coefficient of amino acid in pine seed protein, water-soluble protein（82.9256） 〉 alkali-soluble protein（g1.453） 〉 Gliadin（79.0838 ） 〉 Total protein（77.1625） 〉 salt -soluble protein（46.3652）.The first limiting AA was Val in total amino acid,water-soluble protein and salt-soluble protein.The first limiting AA was Cys-Met in alkali-soluble protein and Gliadin.