刺槐根系可溶性蛋白SDS-PAGE分离方法的改进

Methodological Improvement of Seperating Soluble Protein by SDS-PAGE from Robinia pseudoacacia Root

在利用常规SDS-PAGE方法分离刺槐根系可溶性蛋白的过程中,将样品处理液中的SDS、β-巯基乙醇、丙三醇的含量均增加50%。这样改进后的方法得到了分离效果明显较常规方法好的电泳图谱。经分析得出:常规SDS-PAGE方法中样品处理液的SDS和β-巯基乙醇浓度不足以充分溶解刺槐根系中的可溶性蛋白,丙三醇的浓度也不足以将样品很好地沉聚于点样槽底部。该试验为植物组织可溶性蛋白的SDS-PAGE分离提供了一套效果较好的参考方法。

An improvement was made in the conventional method SDS-PAGE for separating soluble protein from the roots of Robinia pseudoacacia by increasing the content of SDS, β-mercaptoethanol and glycerine to 50%. The separating effect of improved method was much better than the conventional one. Causes of the improvement were discussed; in the conventional method, the concentrations of SDS and β-mercapto- ethanol were not high enough to completely dissolve the soluble protein, and the concentration of glycerine was also not high enough to precipitate the samp e,