固相膜萃取-超高效液相色谱-荧光法测定极地水体中多环芳烃

Determination of polycyclic aromatic hydrocarbons in water from polar regions by solid-phase membrane extraction and ultra performance liquid chromatography with fluorescence detector

采用C18固相膜萃取对样品进行富集净化,以二氯甲烷洗脱目标化合物,采用UPLC荧光可变波长进行分离分析。可在5min内实现15种多环芳烃分析,方法检出限分别为:萘为0.3ng/L,苊、芴、菲和苯并(a)蒽为0.26ng/L,蒽、荧蒽、苯并(b)荧蒽和茚并(1,2,3-cd)芘为0.28ng/L;芘、屈、苯并(k)荧蒽、苯并(a)芘和二苯并(a,h)蒽为0.24ng/L;苯并(g,h,i)苝为2.6ng/L。加标回收率在67%～87%之间,RSD均小于10%。可应用于极地环境中痕量多环芳烃样品的检测分析。

The water samples were enriched and separated by solid-phase membrane.PAHs were eluted by methylene dichloride and determined by UPLC with different fluorescence wavelengths.Fifteen kinds of PAHs were all detected in five minutes.The method quantification limits were 0.3 ng/L for Nap,0.26 ng/L for Acp,Fl,Phe and BaA,0.28 ng/L for An,Flu,BbF and Bghip,0.24 ng/L for Phy,Chr,BkF,BaP and DbA,and 2.6 ng/L for InP.The recoveries were 67%～87%.The relative standard deviations were all less than 10%.This method was handy,sensitive,accurate and suitable for the determination of PAHs according to the results of practical samples,especially samples at trace level.It provided a reliable method for the study of PAHs in polar region.