茜素红与头孢哌酮的荷移反应研究与应用

Study and application of charge transfer reaction between alizarin red S and cefoperazone

实验以茜素红(ARS)为荷移试剂,采用分光光度法研究了ARS与头孢哌酮(CPZ)形成络合物的光谱性质及其反应的适宜条件。实验结果表明:头孢哌酮在2.0～200μg/mL范围内遵循比尔定律,回归方程为:A=0.0036ρ+0.000095(:ρμg/mL),检出限0.75μg/mL,表观摩尔吸光系数2.64×104L·mol-1·cm-1,相关系数为r=0.9965,并初步探讨了反应机理。用实验方法对尿样及生物样品进行加标回收测定,回收率在95.4%～104.8%。

The visible spectral characteristics and the reaction conditions of complex of cefoperazone（CPZ） and alizarin red S（ARS） were investigated by spectrophotometry with ARS as a chromogenic reagent.The results showed that Beer＇s law was obeyed in the concentration range of 2.0～200 μg/mL for CPZ and the regression equation was A=0.0036ρ＋0.000095（ρ：μg/mL） with the correlation coefficient R of 0.9965,the apparent molar absorptivity was 2.64×104 L·mol-1·cm-1 and the detection limit was 0.75 μg/mL.Furthermore,the reaction mechanism was also explored elementarily.The proposed method has been successfully applied to the determination of CPZ in urine and biological samples,and the results were satisfying.