中性红与透明质酸钠相互作用的共振瑞利散射光谱及其分析应用

Resonance rayleigh scattering spectrum of interaction of sodium hyaluranate with neutral red and its analytical application

在pH4.3～5.2的B-R缓冲溶液中,中性红与透明质酸钠作用形成离子缔合物时导致溶液共振瑞利散射(RRS)大大增强并产生新的RRS光谱,其最大散射峰位于328nm处,另在605nm处有一个较弱的散射峰。透明质酸钠质量浓度在0～2.5mg/L范围内,与RRS强度有良好的线性关系。据此,建立了新的测定透明质酸钠的分析方法。该法的检出限(3σ)为25.9ng/mL,并已用于滴眼液和化妆水中透明质酸钠的测定。

In a buffer medium of pH 4.3～5.2,a compound complex was formed between sodium hyaluranate（SH） and neutral red（NR）,leading to a great enhancement of the intensity of resonance rayleigh scattering（RRS） and giving a new RRS spectrum.The maximum peak is located at 328 nm and a relatively weak peak is located at 605 nm.It was also found that the intensity of RRS was directly proportional to the concentration of SH in the range of 0～2.5 mg/L.Based on these facts,a sensitive method for the determination of SH was proposed.The detection limit（3σ） of this method was found to be 25.9 ng/mL.Good selectivity of the method was shown by the results of interference test.The proposed method has been used for the determination of total amounts of sodium hyaluranate in samples with satisfactory results.