苦瓜水提物对实验性糖尿病小鼠血糖、血脂的影响

Effects of Water Extract from Momordica charantia L.on Serum Glucose and Lipids of Experimental Diabetic Mice

目的:研究苦瓜水提物对实验性2型糖尿病模型小鼠体重和血清中血糖值(GLU)、总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)和游离脂肪酸(NEFA)的影响。方法:雄性昆明小鼠喂以高脂饲料(10%蛋黄,10%猪油,1%胆固醇,0.2%胆盐,78.8%基料)1个月,诱发胰岛素抵抗,继以低剂量四氧嘧啶(90 mg·kg^(-1),腹腔注射),使之产生高血糖症,建立2型糖尿病小鼠模型。以苦瓜水提取物10 g·kg^(-1)(相当于生药材重量)的剂量通过灌胃给药,30 d后测定小鼠空腹血糖、糖耐量、血液各项生化指标。结果:显著降低了2型糖尿病小鼠的GLU、NEFA、TC、TG和MDA值(P<0.05);显著升高了2型糖尿病小鼠SOD值(P<0.05)。结论:苦瓜水提物能够显著降低2型糖尿病小鼠的血糖值,并可调节其血脂代谢。

Objective： To investigate the effects of water extract from Momordica charantia L. on the levels of GLU,TC,TG, SOD, MDA and NEFA in blood of alloxan-induced type- Ⅱ diabetic mice. Method： The insulin resistance in mice was induced by fed with high-fat diet （10% yolk, 10% lard, 1% Cholesterol,0. 2% bile salt and 78. 8% base stock） for one month followed by intraperitoneal injection of alloxan at 90 mg · kg^-1 to make the type- Ⅱ diabetic mice. The model mice were administered with water extract from Momordica charantia L. at the dosage of 10 g· kg^-1. The levels of fasting blood glucose, glucose tolerance and other blood biochemical indices were determined after administration of the extract for 30 days. Result： Water extracts from Momordica charantia L. at the dosage of 10 g· kg^-1 could significantly reduce the levels of GLU, TC ,TG and MDA （P 〈 0. 05 ） ; and significantly increase SOD （P 〈 0. 05 ） in alloxan-induced type-Ⅱ diabetic mice. Conclusion： Water extract from Momordica charantia L. can significantly reduce blood glucose and improve lipid metabolism of alloxan-induced type- Ⅱ diabetic mice.