摘要
目的建立川芎嗪聚乳酸纳米粒包封率测定方法。方法采用Hypersil ODS2(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水(52∶48),流速为1 mL/min,柱温:30℃,检测波长为279 nm的色谱条件,低温超速离心法测定包封率。结果该方法可以用于测定川芎嗪聚乳酸纳米粒的包封率。
OBJECTIVE To develop a method for determining the entrapment efficiency of tetramethylpyrazine polylactic aci nanoparticles.METHODS The determining was performed by HPLC with Hypersil ODS2（250mm×4.6mm,5μm） conlumn at 30℃.The mobile phase was methanol-water（52：48）,the flow rate 1.0 ml/min and the UV detection wavelength 279 nm.Ultracentrifugation method with low temperature was used to determine the entrapment efficiency of tetramethylpyrazine polylactic aci nanoparticles.CONCLUSION The designed method can be used for determining the entrapment efficiency of tetramethylpyrazine polylactic aci nanoparticles.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2011年第1期77-79,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省自然科学基金(BK2008459)
南京中医药大学重点培育基金(09XPY05)
关键词
川芎嗪
聚乳酸
纳米粒
包封率
tetramethylpyrazine
polylactic aci
nanoparticles
entrapment efficiency
