HPLC法测定牛黄消炎片中大黄酚的含量

Determination of the Chrysophanol in the Nuihuangxiaoyanpian Tablet by HPLC

目的:建立牛黄消炎片中大黄酚的含量测定方法。方法:采用高效液相色谱法,色谱条件:色谱柱为Diamonsil(TM钻石)(C185μm,4.6mm×250mm);流动相:甲醇-0.1%磷酸溶液(85:15);检测波长为:430nm;柱温:25℃;流速:1.0ml/min;理论板数按大黄酚峰计算不低于3000。结果:大黄酚在0.103～1.030μg之间线性关系良好,r=0.9998。大黄酚平均回收率为98.20%,RSD=0.89%。结论:该方法准确,重复性好,可用于牛黄消炎片的质量控制。

Objective：To establish the quantitative method of the Chrysophanol in the Nuihuangxiaoyanpian Tablet by HPLC.Methods：HPLC.Chromatographic Condition：The Diamonsil（TM钻石） C18 Column（250×4.6 mm,5 μm） was used,The methanol-1%phosphoric acid（V：V=85：15） mixture as the mobile phase,with the detection at 430 nm,The flow rate was 1.0 ml/min,The column temperature was 25℃,The theoretical plates of the Chrysophanol＇s peak was more than 3000.Result：The liner ranges are 0.103-1.030 μg,r=0.999 8.The mean recovery of Chrysophanol was 98.20%,RSD=0.89%.Conclusion：This method is accurate、reproductivity and can be used for the Nuihuangxiaoyanpian Table＇s quality control.