捕食线虫真菌Drechslerella dactyloides Gα基因克隆及系统发育分析

Gene cloning and phylogenetic analysis on the Gα from nematode trapping fungus Drechslerella dactyloides

运用简并PCR和DNA Walking技术首次从捕食线虫真菌(Drechslerella dactyloides)中克隆了Gα的编码全长基因Dga1.该基因全长1 420 bp,编码356个氨基酸,推测其编码蛋白的分子质量和pI值分别为40.84ku和6.68.通过同源比对和系统发育分析,表明Dgal属于真菌Gα的第3类群,推测Dgal具有激活腺苷酸环化酶的作用.

Dgal,a gene encoding Gα,was firstly cloned from nematode-trapping fungus Drechslerella dactyloides by using methods of degenerate PCR and DNA Walking.The Dgal comprised 1 402 base pairs in length,and encoded a polypeptide with 356 amino acid residues.The theoretic molecular mass and pI of Dgal are deduced as 40.84 ku and 6.68,respectively.A phylogenetic analysis on fungal Gα indicates that the Dgal belongs to group Ⅲ of fungal Gα,which possibly has the function as stimulating the adenylyl cyclases.