微孢子虫及人芽囊原虫混合感染与病原体形态观察

MIXED INFECTION OF MICROSPORIDIA AND BLASTOCYSTIS HOMINIS AND THE OBSERVATION ON MORPHOLOGY OF THE PATHOGENS

目的鉴别微孢子虫和人芽囊原虫及其各发育阶段的形态学特征，探讨引起腹泻的相关病因。方法取病人排泄物涂片进行活体观察；将涂片固定染色制成永久性标本；对从排泄物中分离的脱落肠组织进行切片，采用光学显微镜和透射电镜观察及图像分析。结果观察到该两种病原体不同发育阶段的外部形态特征及内部基本结构，初步确定了其属性及寄生关系。结论微孢子虫和人芽囊原虫的不同发育阶段寄生于肠组织，其所造成的肠组织坏死是引起慢性腹泻的主要原因。

Aim To identify the morphologic features of WTBXMicrosporidia and Blastocystis hominis with different developing periods,and to explore the related pathogenesis caused the diarrhea. Method The living pathogens of the smears from patients' excretion were examined by light microscope,which then were fixed and stained as permanent specimens.The dropped intestinal tissue from the excretion were sliced and observed by light microscope,transmission electron microscope and picture-analysis. Results The morphologic features and the internal structure of the pathogens have been observed.The character of the pathogens and its parasitism have been preliminarily defined. Conclusion It showed Microsporidia and Blastocystis hominis living in intestinal tissue with different developing periods,which leads to the necrosis of the tissue,and causes chronic diarrhea.