IFAT检测登革热IgM抗体的初步探讨

PRELIMINARY STUDY ON DETECTION OF IGM ANTIBODY IN DENGUE FEVER BY INDIRECT IMMUNOFLUORESCENCE ASSAY TEST

目的探讨用间接免疫荧光试验（ＩＦＡＴ）检测登革热ＩｇＭ抗体。方法采集１９９５年７～１１月份广东省登革热Ⅰ型流行期间疑似患者发病１～７ｄ血清６２份，用ＩＦＡＴ检测登革热ＩｇＭ抗体。结果阳性率为２４．１９％（１５／６２），发病４～５ｄ的阳性率最高为４０．００％以上。ＩｇＭ阳性滴度在１∶１０～１∶４０之间，多数为１∶１０（８／１５）。本法与乙脑血清有交叉反应，与其他非登革热血清无交叉反应。在发病５～６ｄ的患者中可同时检测出登革热的ＩｇＭ和ＩｇＧ抗体。结论发病１～３ｄ的血清可进行病毒分离，４～６ｄ的血清可检测ＩｇＭ抗体，如阴性则再检测ＩｇＧ，以提高登革热的检出率。

Aim Research about the detection of IgM antibody in patients wioh Dengue fever with by IFAT. Method During dengue fever epidemics(serotype ) occurred in Guangdong Province from July to November 1995.62 sera were collected within 17 days of disease onset from the suspected patients for detection of dengue fevers IgM antibody by IFAT. Result The positive rate was 24.19%(15/62) and the highest rate was in 45 days of diseases onest with positive rate40.00%.The IgM titer was between 110140,and most of them was 110(8/15).The crossreactions was found in sera from the patients with encephalitis,but not from other nondenguefever sera.IgM and IgG antibodies could bedetected at the sametime from the 56 th day sera. Conclusions The sera within 13 days of disease onset can be used for virus isolation,and between 46 days for IgM detection.If negative IgG detection should be futher carried on,so as to improve dengue fever detectoin rate