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Cre/loxp重组系统在转基因植物中删除标记基因的应用 被引量：1

Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants
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摘要 在植物遗传转化中,标记基因是必须的,但标记基因的存在引起人们对转基因食品安全性的关注。Cre/loxp重组系统可用于删除转基因植物中的标记基因,可以通过杂交或二次转化,或瞬时表达、诱导表达、组织特异表达重组酶来获得无标记基因的转基因植株。 Seleetable marker genes are necessary, for plant genetic transformation , but cause attentions about the security of genetically modified food . To remove selectable marker genes from transgenic plants by Cre/loxp recombination system, there are generally several strategies ： crossing with Cre transgenic plant or constitutive expression of recombinase by re - transformation of Cre gene,or application of transient,inducible or tissue - specific expression of recombinase.
作者 郑雪琴
机构地区 福建师范大学生命科学学院
出处 《安徽农学通报》 2011年第1期48-49,68,共3页 Anhui Agricultural Science Bulletin
关键词 Cre/loxp 转基因植物 标记基因 基因删除 Cre/loxp Transgenic plant Selectable marker gene Gene deletion
分类号 Q812 [生物学—生物工程]
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参考文献23

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同被引文献12

  • 1ISAAA发布2010年全球生物技术猫基因作物商业化报告[R].生物技术世界,2011(2): 12-13.
  • 2转基因简介[EB/OL].株洲社科网.http://www. zzsskl.gov. cn/.thread-1657-l. html.
  • 3三转基因大豆获准入华,国内大豆销售或受冲击[EB/OL].新华网.http: //news.xinhuanet.com/fortune/2013-06/14/c_124852477. htm.
  • 4发展转基因作物或可减少温室气体排量[EB/OL].生物 360. http: //www. bio360. net/news/show/4323. html.2013-03-21.
  • 5威廉.恩道尔.中国不需要转基因技术解决吃饭问题[EB/OL].人文与社会.http: //wen.org.cn/modules/ar-ticle/view. article. php/3482/c6.
  • 6美国转基因食品正在透明化其安全性尚未定论[EB/OL].和讯新闻.http: //news, hexun. com/2013-10-21/158896291. html.
  • 7欧洲食品安全局.欧洲食品安全局有关塞拉利尼等研究的最终评价[EB/OL]. http: //www. efsa. europa.eu/en/efsajoumal/pub/2986. htm, 2012-11-28.
  • 8农业部委托进行转基因大米猕猴喂养实验[EB/OL].网易新闻.http: //news.l63.com/13A021/02/9BM5AVJ400014AED. html.
  • 9毛新志.转基因作物产业化的伦理辩护[J].华中农业大学学报（社会科学版）,2011(1):5-11. 被引量：10
  • 10王平安,吴刘记,杨艳坤,吴连成,库丽霞,陈彦惠.转基因植物抗病毒策略及其风险分析[J].河南农业科学,2011,40(2):19-24. 被引量：5

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安徽农学通报
2011年 第1期

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