单克隆抗体对唾液凝集素介导的茸毛链球菌(S.sobrinus)聚集的影响

Effects of Monoclonal Antibody Against the Major Cell Surface Protein on Salivary Agglutinin Mediated Aggregation of Streptococcus sobrinus

目的 :用制备的抗茸毛链球菌主要表面蛋白的单克隆抗体作用于茸毛链球菌 ,体外观察单克隆抗体对唾液凝集素介导的细菌聚集的影响。方法 :于紫外分光光度计上通过光密度值检测 1h内的细菌凝集情况。结果 :单克隆抗体对唾液凝集素介导的茸毛链球菌之间的聚集有一定程度的影响 ,但作用不明显。结论 :该单克隆抗体可能不是对表面蛋白上与聚集作用有关的功能区直接作用。

Objective: To investigate the effects of monoclonal antibody on salivary agglutinin mediated aggregation of Streptococcus sobrinus in vitro. Methods: Bacterial aggregation inhibition was measured under OD\-\{650 at 10 minutes intervals for 1 hour. Results: It showed that the McAb ZS2/286 raised against the PAg of S.sobrinus 6715 could inhibit the aggregation by some degree, but the effect was not significant. Conclusion: The McAb ZS2/286 might not bind directly to the functionally important region of PAg related to aggregation.