性激素及其合成代谢酶在胃癌中的表达及其意义

Expression and significance of sex hormones and their synthetases in gastric carcinoma

目的检测胃癌中雌二醇（E2）、睾酮（T）、17β-羟基类固醇脱氢酶2（17β-HSD2）和17β-羟基类同醇脱氢酶5（17β-HSD5）的表达，探讨其之间的关系及作用。方法应用组织芯片和免疫组织化学技术检测56对样本（胃癌和癌旁正常组织）中E2和T蛋白表达，用逆转录-聚合酶链反应（RT—PCR）方法检测56对样本中17β-HSD2和17β-HSD5 mRNA表达。结果（1）胃癌组织中E2蛋白的相对表达量（0．2381±0．0718）远高于癌旁正常组织（0．1832±0．0628），两者比较差异有统计学意义P〈0．05；与之相反，胃癌组织中17β-HSD2 mRNA的相对表达量（0．8065±0．3797）远低于癌旁正常组织（0．9788±0．2325），两者比较差异有统计学意义（P〈0．05）；在胃癌组织中E2和17β-HSD2之间呈负相关关系（r=0．294，P〈0．05）；（2）胃癌组织中E2蛋白相对表达量有淋巴结转移（0．2532±0．0667）、无淋巴结转移（0．1883±0．0673）间比较和TNM分期中Ⅰ（0．1813±0．0686）、Ⅱ（0．2111±0．0633）、Ⅲ（0．2534±0．0727）、Ⅳ（0．2595±0．0540）间比较均差异有统计学意义（P〈0．05）；（3）胃癌组织中17β—HSD2 mRNA相对表达量男（0．8645±0．3415）及女（0．6145±0．4479）之间、分化好（0．6717±0．4182）、分化差（0．8714±0．3613）间比较差异均有统计学意义（P〈0．05）。结论胃癌发生发展与E2蛋白表达增强和17β-HSD2 mRNA转录减弱明显相关。

Objective To investigate the expression of estradiol （ E2 ） , testosterone （T） , estrogen receptor （ER）, androgen receptor （AR）, 17β-hydroxysteroid dehydrogenase2 （17β-HSD2） and 17β- hydroxysteroid dehydrogenase5 （17β-HSD5） in gastric carcinomas and their relationship. Methods Tissue microarray technology and innnunohistochemical Elivision plus method were used to detect the expression of E2, T protein in 56 pairs of samples of gastric carcinoma and normal gastric tissue. The mRNA transcription levels of 17β-HSD2 and 17β-HSD5 were measured by reverse transcription-polymerase chain reaction （RT-PCR）. Results （ 1 ） The E2 protein expression levels in gastric carcinoma tissues （0. 2381 ± 0. 0718） was significantly higher than in normal gastric tissues （0. 1832 ±0. 0628, P 〈 0. 05 ）, on the contrary, the 17β-HSD2 mRNA transcription level in gastric carcinoma tissues （0. 8065 ± 0. 3797） was significantly lower than in normal gastric tissues （0. 9788 ±0. 2325 ,P 〈0. 05）, a negative correlation between them （r = 0. 294, P 〈 0. 05 ） ; （2） The E2 protein level was higher in gastric carcinoma tissues with lymph node metastasis （0. 2532 ± 0. 0667 ） than in those without lymph node metastasis （0. 1883 ± 0. 0673 ,P 〈0. 05）. The expression of E2 protein in TNM stages Ⅰ, Ⅱ ,Ⅲ, Ⅳ respectively was （0. 1813 ±0.0686）, （0.2111 ±0.0633）, （0.2534 ±0.0727）, （0.2595 ±0.0540）; （3） The 17β-HSD2 mRNA transcription level was higher in gastric carcinoma tissues of men （0. 8645 ± 0. 3415） than in those of women （0. 6145 ± 0. 4479 ,P 〈 0. 05 ） and the same positive relationship was observed between the well differentiation and the poorer differentiation. Conclusion The tumorigenesis and progression of gastric carcinoma has an important correlation with up-regulated E2 protein expression level and down-regulated 17β-HSD2 mRNA transcription level.