氟哌酸胶囊溶出度测定方法的试验

Study on the method of dissolution test of norfloxacin Capsule

目的：探讨紫外分光光度法测定氟哌酸胶囊含量的条件、方法和氟哌酸胶囊溶出度的测定。方法：用紫外分光光度法测定氟哌酸胶囊含量及转篮法测定其溶出度。结果：在２７３ｎｍ 波长处有最大吸收（５μｇ／ｍｌ，０４ ％ 氢氧化钠溶液） ，其吸收系数平均值（ Ｅ１ ％１ ｃｍ ） 为１０９５ ， 线性关系良好（ ｒ ＝０９９９８） 。２０ｍｉｎ 溶出率（ ％ ） 均在８０ ％ 。结论：氟哌酸胶囊含量测定可采用紫外分光光度法，简便易行，快速准确。同时可用于氟哌酸胶囊溶出度测定。

OBJECTIVE: To study the condition and method using UV-spectrophotomery for determining the concentration and dissolution of Norfloxacin capsule.METHODS:The concentration of Norfloxacin capsule was detected by UV-spectrophotometry and the dissolution was detected by rotation basket method.RESULTS:The iggest absorbtion was at 273nm wavelength(5μg/ml,0.4% NaOH solution).The average absorption coefficient( E 1% 1cm )is 1095,And the linear relationship was good( r =0 9998)In 20minutes,the dissolution rates were 80%.CONCLUSION: The concentration of Norfloxacin can be detected by UV-spectrophotometry,it′s convient and rapid.It can also be wsed to detect the dvssolution of Norfloxacin capsule.