丁基苯酞对缺血脑组织中及低糖低氧刺激后培养的神经细胞中胆碱乙酰化酶活性的影响

Effect of dl3nbutylphthalide on the activity of the choline acetyltransferase in ischemic brain and cultured neurons subjected to hypoglycemia/hypoxia

目的：观察丁基苯酞（ｄｌＮＢＰ），ｄＮＢＰ和ｌＮＢＰ对脑缺血后皮层和纹状体中以及低糖低氧和ＮＭＤＡ刺激后培养的胎鼠皮层神经元中胆碱乙酰化酶活性变化的影响。方法：大脑中动脉堵塞起始处造成大鼠局灶性脑缺血；胎鼠皮层神经元以低糖低氧培养基培养造成低糖低氧模型；胆碱乙酰化酶活性以分光度法进行测定。结果：脑缺血后缺血区皮层和纹状体中胆碱乙酰化酶活性分别下降了６１．３％和５８．４％。ｄｌＮＢＰ，ｄＮＢＰ和ｌＮＢＰ（１０和２０ｍｇ·ｋｇ－１，ｉｐ）于脑缺血后５和６０ｍｉｎ给药２次皆可显著提高脑组织中胆碱乙酰化酶活性。ｄｌＮＢＰ，ｄＮＢＰ和ｌＮＢＰ（０．１～１０μｍｏｌ·Ｌ－１）对低糖低氧及ＮＭＤＡ刺激后神经细胞中胆碱乙酰化酶活性也有明显的提高作用。结论：ｄｌＮＭＰ改善局灶性脑缺血后动物的学习记忆功能可能与其对胆碱能神经功能的改善有关。

OBJECTIVE: To investigate the effects of dl3nbutylphthalide (dlNBP),dNBP and lNBP on the activity of choline acetyltransferase (ChAT) in ischemic brain after focal cerebral ischemia and in neurons subjected to hypoglgcemia/hypoxia. METHODS: Focal cerebral ischemia was induced by blockade of the origin of middle cerebral artery (MCAO) and perinatal cortical rat neurons was cultured with hypohlycemia/hypoxic medium to induce hypoglycemia/hypoxia injury.The activity of ChAT was determined spectrophotometrically. RESULTS: The results indicated that the activity of ChAT was decreased by 61.3% and 58.4% respectively in cerebral cortex and striatum after six hours of MCAO.dlNBP,dNBP and lNBP (10 and 20mgkg-1,ip) were shown to increase ChAT activity in ischemic brain significantly.The decreased activity of ChAT in cultured perinatal rat cortical neurons induced by hypoglycemia/hypoxia or by NMDA was also improved by dlNBP,dNBP and lNBP (0.110molL-1). CONCLUSION: The effect of dlNBP on learning and memory function impaired by focal cerebral ischemia may be related to its protective effect on the activity of choline acetyltransferase.