荧光原位杂交技术检测尿液脱落上皮细胞染色体畸变对膀胱癌的诊断价值

Diagnostic value of chromosome aberration of urine exfoliated cells by fluorescence in situ hybridization for bladder carcinoma

目的探讨应用荧光原位杂交（FISH）技术检测尿液脱落上皮细胞3、7、9、17号染色体畸变对于膀胱癌诊断的价值。方法分别对30例膀胱癌患者和20例非肿瘤血尿患者应用FISH试剂盒检测膀胱癌，使用荧光标记的DNA探针与3、7和17号染色体的着丝粒区以及9p21位点相结合，检测尿路上皮细胞基因的改变。在组织学诊断的基础上评估FISH检测的价值。结果30例膀胱癌患者FISH检测的特异性和敏感性分别为100％和97％。结论FISH是一种无创的膀胱癌诊断方法，具有快速、准确、灵敏度高的优点，对膀胱癌的诊断具有重要的临床应用价值。

Objective To evaluate the diagnostic value of chromosome aberration of urine exfoliated cells by fluorescence in situ hybridization （FISH）for patients with bladder carcinoma. Methods Using centromeric probes of chromosomes 3, 7, 17 and region probe of 9p21, we performed FISH on the exfoliative cells in the urinary samples from 30 patients with pathologically confirmed bladder .carcinoma and 20 hematuria patients with benign bladder disease controls. Then we noted and analyzed aberrations in the chromosomes. Results The sensitivity and specificity of FISH on detecting bladder cancer were 97% and 100% , respectively. Conclusions It is noninvasive and effective to diagnose bladder cancer with the techiqtre of FISH. It is a rapid, accurate and very sensitive method and can be used in clinical diagnosis.