心肌缺血／再灌注期间硒拮抗氧自由基产生的研究

Study on the antagonizing effect of selenium to oxide free radical during cardiac muscle ischemia/reperfusion

目的：探讨补硒后硒拮抗氧自由基产生的效果及其对某些微量元素的影响。方法：用硫代巴比妥酸法测心肌丙二醛（ＭＤＡ）含量，用二硫代二硝基苯甲酸法（ＤＴＮＢ）测谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活性，用石墨炉法测Ｓｅ含量，用火焰法测Ｍｇ、Ｃａ含量。结果：补硒后大鼠心肌ＧＳＨＰｘ，Ｓｅ，Ｍｇ含量均有所升高，ＭＤＡ，Ｃａ有所降低。有机硒组主要影响ＧＳＨＰｘ，Ｃａ；无机硒主要影响Ｍｇ，以再灌注期更明显。结论：补硒后通过升高心肌ＧＳＨＰｘ活性、Ｍｇ含量，减轻Ｃａ超载，可能是抗自由基损伤的重要机制。

BJECTIVE: To investigate the antagonizing effect of selenium (Se) to the production of oxide free radical and the impact to some trace elements after Se taken METHODS: To determinate of MDA contents by TBA,GSHPx activity by DTNB,Se content by graphite furnace assay,Mg2+ content and Ca2+ content by flame assay,respectivelyT5HZRESULTS: Rat cardiac muscle GSHPx activity,Se2+ and Mg2+ were increased after Se taken,while MDA and Ca2+ contents were decreased.GSHPx and Ca2+ were mainly influenced in organic Se group,while Mg2+ was mainly influenced in inorganic Se group,especially during reperfusion period. CONCLUSION: The increase of GSHPx activity and Ca2+ content and the decrease of Ca2+ through Se taken may be involved in the mechanism of Se antagonizing to free radical damage in cardiac muscle in rats.