高效液相色谱法测定血浆中泮托拉唑浓度

Determination of pantoprazole in plasma by HPLC

目的：建立用高效液相色谱法测定血浆中泮托拉唑浓度的方法，为进行泮托拉唑的体内药物代谢、生物利用度研究提供基础。方法：血浆用０．０１ｍｏｌ·Ｌ－１磷酸氢二钠做ｐＨ调节剂，二氯甲烷做提取溶剂，硝西泮作内标。采用ＰｌａｔｉｎｕｍＣ１８（５ｕ，２５０ｍｍ×４．６ｍｍ）柱，流动相：甲醇水醋酸盐缓冲液（４０∶６０∶５），流速１．０ｍｌ·ｍｉｎ－１，检测波长２８６ｎｍ。结果：该法回收率平均在９８．３６％～１０４．２７％，重现性高，ＲＳＤ在４．６９３％～９．１２％范围内（ｎ＝５），线性范围：２０～４０００μｇ·Ｌ－１，最低检测浓度１５μｇ·Ｌ－１。结论：ＨＰＬＣ测定血浆中泮托拉唑的方法简便、准确、灵敏、专一，能满足血药浓度测定及药代动力学研究的需要。

BJECTIVE: To establish an assay to determine pantoprazole in plasma by means of highperformance liquid chromatography for the pharmacokinetic study of pantoprazole. METHOD: The platinum C18 column(250mm4.6mm,5u) was used as analytical column.The mobile phase consisted of methanolwateracetate buffer(pH4.3)40605.The flow rate was 1.00mimin-1.The blood samples were extracted by 0.01molL-1Na2HPO4 solution and dichloromethane,=286nm. RESULTS: The recovery of this method was 98.36%104.27% and the validation was RSD=4.69%9.12%(n=5). CONCLUSION: The method is simple, accurate and sensitive.It provides a suitable analytical method for its pharmacokinetic studies.