3个紫花苜蓿品种耐盐突变材料的耐盐性评价

Evaluation on salt tolerance to salt-tolerant variant materials of three alfalfa varieties

选取国外耐高温高湿的3个紫花苜蓿(Medicago sativa)品种:"维多利亚"、"盛世"和"CW787",用NaCl作选择剂,采用一步和多步正筛法,筛选出耐盐愈伤组织变异系,对其再生植株的丙二醛、脯氨酸、可溶性蛋白和可溶性糖含量,以及超氧化物歧化酶活性等生理指标进行了测定,并采用隶属函数法对耐盐突变材料的抗盐性进行了综合评价。结果表明,经筛选得到的耐盐突变材料的丙二醛、脯氨酸、可溶性蛋白和可溶性糖含量以及超氧化物歧化酶活性均显著(P<0.05)高于未经筛选的对照植株,其耐盐性大小依次为:"盛世">"CW787">"维多利亚"。

Three foreign alfalfa （Medicago sativa） varieties （Victoria, Millennium, and Cw787） with strong resistance to high temperature and humidity were selected to obtain salt-tolerant callus variants by using sodium chloride （NaCl） as selective reagent via positive selection （direct selection and continuous selection） methods. The physiological indexes, including MDA, proline, soluble protein and soluble sugar content, as well as superoxide dismutase （SOD） activity of regeneration plants of callus variants were measured and these physiological indexes was used to comprehensively evaluate the salt tolerance by Subordinate Function method. The results of this study showed that the MDA, proline, soluble protein and soluble sugar content, as well as superoxide dismutase （SOD） activity of regeneration plants of callus variants were significantly （P〈0.05） higher than that of control plants. The order of salt tolerance was Millennium 〉 CW787〉 Victoria.