ACE基因插入/缺失多态性与脑血管病的关联性研究

A Study on relationship between Insertion/Deletion Polymorphism of Angiotensin-Converting Enzyme Gene and Cerebrovascular Disease

目的:探讨血管紧张素转换酶(ACE)基因插入(I)/缺失(D)多态性与脑血管病(CVD)的关系。方法:采用聚合酶链反应技术(PCR)检测19Z例卒中患者、95例高血压病人和124例正常人的ACE基因多态性。结果:CVD组ACE基因D等位基因频率为0.58,明显高于高血压对照组(P＜0.02)和正常对照组(P＜0.01),DD基因型频率明显高于正常对照组(P＜0.05)。腔隙性梗死(LACI)组的DD基因型显著高于对照组(P＜0.05)。多元回归分析发现ACE*DD基因型与卒中无明显相关性(P＜0.08),而与LACI存在明显相关性(P=0.048)。结论:ACE基因缺失多态性在LACI的形成中可能产生重要作用。

Aim: To study the relationship between the angiotensin-converting enzyme (ACE) inser-tion(I)/deletion(D) polymorphism and cerebrovascular disease. Methods: We identified the ACE gene polymorphisms by PCR in 192 stroke subjects, 95 hypertensive and 124 normotensive control subjects. Results:D allele frequency (0. 58) in stroke group was significantly higher than in hypertensive (P<0. 02) and normotensive control group(P<0. 01). ACE DD genotype in stroke group was also more common than in normal control group(P<0. 05). DD genotype in lacunar infarction was significantly higher than in control group(P <0. 05). Deletion polymorphism of ACE gene was significantly associated with lacunar infarction (P = 0. 048), but not with general stroke (P=0. 08) by multiple regression analysis. Conclusion: Deletion polymorphism of ACE gene may play an important role in development of lacunar infarction.