神经干细胞与嗅鞘细胞联合移植治疗大鼠脑外伤的实验研究

Treatment of traumatic brain injury in rats with neural stem cells and olfactory ensheathing cells transplantation

目的探讨神经干细胞与嗅鞘细胞联合移植对大鼠创伤性脑外伤的治疗作用。方法 125只SD成年大鼠随机分为损伤对照组(A组)、神经干细胞移植组(B组)、嗅鞘细胞移植组(C组)、神经干细胞嗅鞘细胞联合移植组(D组)、假手术组(E组)。各组均咬开骨窗,显露硬膜,除假手术组外,各组均采用经典的Feeney氏法自由落体撞击法造成创伤性脑外伤。于造模后1、14、30d进行神经功能评分并取材行HE及免疫组化染色。结果 125只大鼠均进入分析,B组、D组14、25、30d较A组、C组差异有统计学意义(P<0.05),B组、C组、D组行为学评分在1d后各时间点与A组比较差异有统计学意义(P<0.05),D组在25、30d与B组比较差异有统计学意义(P<0.05)。结论嗅鞘细胞联合神经干细胞移植有助于治疗大鼠创伤性脑外伤。

Objective To study the effect of neural stem cells and olfactory ensheathing cells transplantation on traumatic brain injury in rats.Methods One hundred and twenty-five adult SD rats were randomly divided into control group（group A）,neural stem cells transplantation group（group B）,olfactory ensheathing cells transplantation group（group C）,neural stem cells and olfactory ensheathing cells transplantation group（group D）,sham operation group（group E）,25 rats in each group.Bone window was opened to expose the dura matter.Traumatic brain injury was induced using the Feeney′s method of free-fall impact in the other 4 groups except for sham operation group.Neurological function was scored 1,14 and 30 days,respectively,after injury was induced.Samples were taken and stained with HE and immunohistochemistry.Results The therapeutic effect was better in groups B and D than in groups A and C 14,25 and 30 days after operation（P0.05）.The neurological function score was higher in groups B,C and D than in group A 1,25 and 30 days after operation（P0.05）.Conclusion Neural stem cells and olfactory ensheathing cells transplantation can treat traumatic brain injury in rats.