摘要
根据腐蹄病节瘤拟杆菌毒力菌株具有溶解弹性硬蛋白的特性，利用弹性硬蛋白培养基建立了弹性硬蛋白溶解试验，以鉴别节瘤拟杆菌强毒株。试验表明，将不同毒力的菌株同时接种于弹性硬蛋白培养基，蛋白溶解带出现的时间和宽度都显著不同，强毒株大多在７ｄ内，少部分在７～１１ｄ内均出现一条明显的蛋白溶解带；而弱毒株在２１ｄ内基本上未见蛋白溶解带出现。
The experimental results showed that cultures of virulent isolates invariably produced elastase and solubilized elastin in medium after 7 or 711 days'incubation,whereas cultures of benign isolates were elastase negative until 21 days'incubation,which means there was apparent correlation between elastase activity and virulence.
出处
《经济动物学报》
CAS
1999年第2期54-57,共4页
Journal of Economic Animal
基金
"九五"国家攻关课题
