摘要
Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.Methods: Cells proliferation was assessed by MTT assay,cells morphologic was observed by the inverted microscopy,Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor cells.The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR.Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in doseand time-dependent ways.After Cinobufacini injection intervention,HepG-2 cells showed typical morphological changes: cells changed from polygon into round,chromatin looseness and karyolysis were observed.The percentages of apoptosis were 88.49%,76.02%,61.73% corresponding to the 48 h interference of 0.42 μg/mL,0.21 μg/mL,0.105 μg/mL Cinobufacini injection,perspectively.RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level.Conclusion: Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis,the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.
Objective： The aim of our study was to investigate the effect of Cinobufacini injection on the proliferat（on and apoptosis of human hepatocarcinoma HepG-2 cells. Methods： Cells proliferation was assessed by MTT assay, cells morphologic was observed by the inverted microscopy, Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor ceils. The expression of TOPOI mRNA and TOPO Ⅱ mRNAwere examined by RT-PCR. Results： Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways. After Cinobufacini injection intervention, HepG-2 cells showed typical morphological changes： cells changed from polygon into round, chromatin looseness and karyolysis were observed. The percentages of apoptosis were 88.49%, 76.02%, 61.73% corresponding to the 48 h interference of 0.42 μg/mL, 0.21 μg/mL, 0.105 μg/mL Cinobufacini injection, perspectively. RT-PCR assay showed that Cinobufacini injection down-regulated TOPOI and TOPO Ⅱ expression at mRNA level. Conclusion： Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis, the mechanism of which might partly relate to the down-regulation of TOPOI mRNA and TOPO Ⅰ mRNA induced by Cinobufacini injection.
