摘要
目的了解各种铬结合物与肝损害的关系。方法在铬中毒性肝损害的大鼠模型中,采用SephadexG50凝胶层析技术,对肝中铬结合物进行分离和测定。结果铬染毒大鼠肝组织中,除可检出高分子铬结合物(HMWCr,相对分子质量(MW)70000~12500)及低分子铬结合物(LMWCr,MW12500~500)外,还发现对照组未检出的小分子铬结合物(MMWCr,MW<500)。随染毒时间延长,肝损害加重,三者肝中分布变化明显:HMWCr由22.2%增至37.0%;MMWCr由2.2%增至5.0%;LMWCr则从75.6%降至58.0%。结论LMWCr可能与铬的解毒有关;MMWCr的出现并增多反映了机体形成LMWCr能力的紧张;MMWCr可能是攻击靶器官的主要活性因素。
Objective To study the role of chromiumbinding
substances in chromiuminduced hepatic damage. Methods A subacute chromium intoxicated
rat model was made and chromiumbinding substances in liver were isolated and measured by
sephadex G50 column chromatography and UVAAS. Results Only a high molecular weight
chromiumbinding substance (HMWCr,MW700 00012 500) and a low molecular weight
chromiumbinding substance (LMWCr,MW12 500500) could be detected in normal animals.But
in chromiumexposed animals,a new minimal molecular weight chromiumbinding substance
(MMWCr,MW<500) was found in addition to the above constituents.As the exposure time
prolonged,hepatic damage aggravated and the distribution of three Crbinding substances was
as follows:HMWCr from 22.2% to37.0%,MMWCr from 2.2% to 5.0%,LMWCr from 75.6% to 58.0%.
Conclusion LMWCr play a main role in chromium detoxification.The appearance and increase of
MMWCr reflected on the insufficiency of LMWCr formation.The MMWCr should be a major
active factor on pathogenesis of Crintoxication.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1999年第3期164-167,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金
关键词
铬中毒性
肝损害
铬结合物
Hepatotoxic damage by
chromiumChromiumbinding substances