摘要
从湖南石门县及郴州市重金属矿周边地区采集的6个砷污染土壤样品中,初步分离得到13株具有较强耐砷能力的真菌菌株,其中3株真菌的耐砷能力最强,经形态与分子鉴定分别为微紫青霉(Penicillin janthinellum)、尖孢镰刀菌(Fusarium oxysporum)和棘孢木霉(Tri-choderma asperellum).培养试验表明:微紫青霉、尖孢镰刀菌和棘孢木霉分别在30000mg·L-1、30000mg·L-1和20000mg·L-1的砷胁迫平板中表现出很好的菌落生长状况;在砷浓度分别为0~50、0~50和0~80mg·L-1的液态培养基中培养2d后,尖孢镰刀菌、棘孢木霉和微紫青霉的生物量均随砷浓度增加而增加,且在相应最高浓度(50、50、80mg·L-1)下的生物量显著高于不加砷的对照.此外,高浓度砷对该3株真菌的产孢能力没有影响.
A total of thirteen fungal strains with higher arsenic (As)-tolerance ability were isolated from six As-contaminated soil samples collected from the mining areas of Shimen County and Chenzhou City in Hunan Province. Among the strains isolated,Penicillin janthinellum,Fusarium oxysporum,and Trichoderma asperellum had the highest As-tolerance ability,based on the morphological identification and phylogenetic analysis. Culture experiment showed that on the solid plates with 30000,30000,and 20000 mg·L -1 of As,P. janthinellum,F. oxysporum,and T. asperellum had a better colony growth,and after cultured in the liquid medium with 0-50,0-50,and 0-80 mg ·L-1 of As for 2 days,respectively,the dry mycelia masses of the three strains all increased with increasing As concentration. When the As concentration reached 50,50,and 80 mg·L -1,respectively,the fungal biomass of F. oxysporum,T. asperellum,and P. janthinellum increased significantly,compared with CK. High concentration As had no significant effects on the sporalation of the three fungal strains.
出处
《应用生态学报》
CAS
CSCD
北大核心
2010年第12期3225-3230,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(40871102)
国家"十一五"科技支撑计划项目(2007BAD89B03)资助
关键词
砷
真菌
分离
耐砷能力
arsenic
fungi
isolation
arsenic-tolerant ability.