肠道缺血-再灌注对肺内源性碱性成纤维细胞生长因子与转化生长因子β表达的影响及其与肺损伤修复的关系

Changes in endogenous basic fibroblast growth factor and transforming growth factor in lung induced by intestinal ischemia reperfusion injury

目的：研究肠道缺血再灌注损伤后肺内源性碱性成纤维细胞生长因子（ｂＦＧＦ）与转化生长因子β（ＴＧＦβ）的变化规律，并探讨这种变化与肺损伤后修复发生的关系。方法：利用肠系膜上动脉夹闭模型，将６０只大鼠分成假手术、缺血４５分钟、缺血４５分钟后再灌注６小时、２４小时与４８小时５组。用ＳＰ免疫组化法研究内源性ｂＦＧＦ与ＴＧＦβ在不同受创条件下肺组织的变化规律。结果：正常肺有少量ｂＦＧＦ与ＴＧＦβ的阳性表达，主要位于肺泡上皮细胞与微静脉内皮细胞。缺血４５分钟与再灌注损伤早期，肺组织ｂＦＧＦ与ＴＧＦβ表达增加，以伤后６小时为甚，以肺泡上皮细胞和毛细血管内皮细胞胞膜最为明显。伤后２４与４８小时随着肺组织结构恢复，两种因子表达量已恢复至正常。结论：肺间接受创后ｂＦＧＦ与ＴＧＦβ两种生长因子表达量增加是对创伤的一种保护性反应，它将有利于机体在受创后的自我修复。

Objective :To explore the alteration of endogenous basic fibroblast growth factor (bFGF) and transforming growth factor (TGF) in lung following intestinal ischemia2]reperfusion injury and its effects to lung repair. Methods :60 Wistar rats were divided into five groups,which were sham operated,ischemia (45 minutes),ischemia (45 minutes)plus reperfusion (6,24 and 48 hours,respectively) groups.Immunohistochemical method was used to observe the localization and amount of both growth factors. Results :Positive immunohistochemical signal of both growth factors could be found in normal lung,mainly in alveolar cells and endothelial cells of vein.After ischemia and reperfusion insult,expression of both growth factors was increased and the amount of expression of both factors at 6 hours was more than those in normal controls or 24 and 48 hours after insult. Conclusions :The results indicate that the endogenous bFGF and TGF expression appears to be up regulated in the lung following intestinal ischemia and reperfusion,suggesting that both of growth factors may be involved in the process of lung repair.