Livin在鼻咽癌中的表达及临床意义

Expression and Clinical Significance of Livin in Nasopharyngeal Carcinoma

目的：探讨鼻咽癌组织中凋亡抑制基因Livin的表达及其与鼻咽癌临床指标间的关系.方法：研究组为鼻咽癌患者56例,其中TNM分期早期患者（Ⅰ期＋Ⅱ期）39例,晚期（Ⅲ期＋Ⅳ期）17例 伴有淋巴结转移36例,无淋巴结转移20例.对照组为鼻咽部慢性炎症患者30例.采用半定量RT - PCR法分析两组之间Livin mRNA的表达情况,以及研究组内不同分期、有无淋巴结转移等情况下Livin mRNA表达的变化.结果：RT- PCR显示鼻咽癌组织中Livin mRNA呈阳性表达（阳性率91.1% ）,而鼻咽部慢性炎症组织中无表达,两组间相对表达量差异有统计学意义（P＜0.05）.研究组中有淋巴结转移组Livin mRNA相对表达量高于无淋巴转移组（P＜0.05） 不同TNM分期患者的Livin mRNA相对表达量,早期患者（Ⅰ期＋Ⅱ期）的阳性表达率为89.7%,明显低于晚期患者（Ⅲ期＋ Ⅳ期）阳性率94.1%,两组间差异有统计学意义（P＜0.05）.结论：Livin在鼻咽癌的发生、发展与转移中扮演重要角色,可能成为鼻咽癌早期诊断的分子指标及分子靶向治疗的新靶点.

Objective ：To investigate the expression and significance of Livin in nasopharyngeal carcinoma （NPC）, and reveal the relationship between NPC pathogenesy and clinical parameters. Methods：56 cases of NPC patients were divided into the early stage group（TNM stage I and Ⅱ ,39 patients） and the advanced stage group（ TNM stage m and Ⅳ, 17 patients）, which consisted of 36 cases with lymph node metastasis and 20 cases without lymph node metastasis. 30 cases of phargnx nasalis chronic inflammation patients were divided in- to the control group. The expression of Livin mRNA in the study group and control group were analyzed by the RT-PCR method. At the same time, comparative analysis of the expression of Livin mRNA was done in different stage, compained by lymphatic metastasis and without lymphatic metastasis. Results：Livin mRNA was strongly amplifieated in the NPC cases （51/56,91. 1% ）, but not in the 30 cases of nasopharyngitis tissues （0/30, 0% ,P 〈 0.05）. The rate of positive expression in early stage （ 1 ＋ Ⅱ ,35/39,89.7% ） was lower than in the advanced stage （ Ⅲ ＋ Ⅳ, 16/17,9,4.1% ,P 〈 0.05 ）. The relative expression levels of Livin mRNA were very different between lymph node metastasis group and nn-metastasis group （ P 〈 0.05 ）. Conclusions ： Livin plays a vital role in the progression of NPC and it is likely to be a molecule parameter in the diagnosis and clinic evalu- ation, and may be a potential target in anticancer of targeted therapy.