蝙蝠葛碱抑制凝血酶诱导的血小板膜糖蛋白Ⅳ再分布和胞内α-颗粒凝血酶敏感蛋白释放(英文)

Dauricine inhibits redistribution of platelet membrane glycoprotein Ⅳ and release of intracellular α-granule thrombospondin induced by thrombin

探讨蝙蝠葛碱（Ｄａｕ）对血小板聚集功能的影响．观察Ｄａｕ对血小板膜糖蛋白Ⅳ（ＧＰⅣ）再分布和胞内α－颗粒凝血酶敏感蛋白（ＴＳＰ）释放的抑制作用．方法：应用流式细胞仪分别检测静息血小板ＧＰⅣ分布，ＴＳＰ释放，活化血小板膜ＧＰⅣ再分布和α－颗粒ＴＳＰ释放．结果： Ｄａｕ并不影响静息血小板膜 ＧＰⅣ和 ＴＳＰ分布，而对活化血小板膜ＧＰⅣ再分布和细胞内α－颗粒ＴＳＰ释放具有明显的抑制作用，且两者的抑制作用呈显著正相关（ｒ＝０．５１１，Ｐ＜０．０１），这种抑制作用并不受Ｃａ２＋浓度的影响．结论：Ｄａｕ抑制活化血小板膜ＧＰⅣ再分布和胞内α－颗粒ＴＳＰ释放。

AIM: To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein Ⅳ (GPⅣ) and release of intracellular α-agranule thrombospondin (TSP) on platelet activation. METHODS: Using the flow cytometric assay of washed platelet to record expression of GPⅣ and release of TSP induced by thrombin. RESULTS: Dan did not affect GPⅣ and TSP on resting platelet membrane but inhibited redistribution of GPⅣ to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPⅣ and TSP (r=0.511, P<0.01 ). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent. CONCLUSION: Dan inhibited redistribution of GPⅣ and release of intracellular α-granule thrombospondin induced by thrombin.