摘要
研究三七总皂苷(PnS)抗炎作用.方法:用角叉菜胶复制大鼠气囊滑膜炎模型.蛋白含量、PLAZ活性、Din含量及炎细胞内游离钙水平分别用Lowry法、微量酸滴定法、放射免疫分析法及 Fura 2-AM荧光分析法进行测定.结果: PnS(60-240 mg· kg-1)明显抑制角叉菜胶诱导的白细胞数升高和蛋白渗出;抑制灌洗液PLA2活性,降低Din含量;阻止炎细胞内游离钙水平的升高.结论:PnS具有明显抗炎作用,其作用机制与其阻止炎细胞内游离钙水平的升高及其抑制PLA2活性、减少Din释放有密切关系.
AIM: To study the anti-inflammatory effects of total saponins of Panax notoginseng (PnS). METHODS: Rat air-pouch acute inflammatory model was established with sc carrageenan (Car, 25mg· kg-1). The-protein content in exudate was measured. Microacid titration assay and redioimmunoassay (RIA) were applied respectiviy to investigate effects of PnS on phospholipase A2 (PLA2) activity and dinoprostone (Din ) content in exudate. Fura-2 fluorescence technique was used to determine the intracellular free calcium concentration in neutrophils (Neu-[Ca2+]i ).RESULTS: At 12 h, PnS 60-240 mg·kg-1 ip reduced Neu counts, protein content [ (7.7±1.3) to(4.4±1.4) g·L-1], and Din content [(1619±391) to (883±268) ng·L-1]; inhibited the PLA2 activity in exudate [(248±42) to (157±35) kU·L-1] in a dose-dependent manner. PnS 60, 120, and 240 mg· kg-1 lowered the level of Neu-[ Ca2+], with the inhibitory rate of 9.1%, 33.2 %, and 39.4% , respectively. CONCLUSION: PnS has an obvious anti-inflammatory effect and its mechanisms are related to the inhibition of the Neu--[Ca2+], level and PLA2 activity, and reduction of Din content.
出处
《中国药理学报》
CSCD
1999年第6期551-554,共4页
Acta Pharmacologica Sinica
关键词
皂苷类
炎症
非甾醇抗炎剂
药理
三七
抗炎作用
ginseng
saponins
inflammation
non-steroidal anti-inflammatory agents
phospholipases A
dinoprostone
calcium
carrageenan
neutrophils
dexamethasone