三七总皂苷的抗炎作用(英文)

Anti-inflammatory effects of total saponins of Panax notoginseng

研究三七总皂苷（ＰｎＳ）抗炎作用．方法：用角叉菜胶复制大鼠气囊滑膜炎模型．蛋白含量、ＰＬＡＺ活性、Ｄｉｎ含量及炎细胞内游离钙水平分别用Ｌｏｗｒｙ法、微量酸滴定法、放射免疫分析法及 Ｆｕｒａ ２－ＡＭ荧光分析法进行测定．结果： ＰｎＳ（６０－２４０ ｍｇ· ｋｇ－１）明显抑制角叉菜胶诱导的白细胞数升高和蛋白渗出；抑制灌洗液ＰＬＡ２活性，降低Ｄｉｎ含量；阻止炎细胞内游离钙水平的升高．结论：ＰｎＳ具有明显抗炎作用，其作用机制与其阻止炎细胞内游离钙水平的升高及其抑制ＰＬＡ２活性、减少Ｄｉｎ释放有密切关系．

AIM: To study the anti-inflammatory effects of total saponins of Panax notoginseng (PnS). METHODS: Rat air-pouch acute inflammatory model was established with sc carrageenan (Car, 25mg· kg-1). The-protein content in exudate was measured. Microacid titration assay and redioimmunoassay (RIA) were applied respectiviy to investigate effects of PnS on phospholipase A2 (PLA2) activity and dinoprostone (Din ) content in exudate. Fura-2 fluorescence technique was used to determine the intracellular free calcium concentration in neutrophils (Neu-[Ca2+]i ).RESULTS: At 12 h, PnS 60-240 mg·kg－1 ip reduced Neu counts, protein content [ (7.7±1.3) to(4.4±1.4) g·L-1], and Din content [(1619±391) to (883±268) ng·L-1]; inhibited the PLA2 activity in exudate [(248±42) to (157±35) kU·L-1] in a dose-dependent manner. PnS 60, 120, and 240 mg· kg-1 lowered the level of Neu-[ Ca2+], with the inhibitory rate of 9.1%, 33.2 %, and 39.4% , respectively. CONCLUSION: PnS has an obvious anti-inflammatory effect and its mechanisms are related to the inhibition of the Neu--[Ca2+], level and PLA2 activity, and reduction of Din content.