肺瘤平膏及其拆方对Lewis肺癌小鼠树突状细胞影响的实验研究

Effect of Feiliuping Ointment and Its Disassembled Prescriptions on Dendritic Cell of Mice with Transplanted Lewis Lung Cancer

目的观察中药复方肺瘤平膏及其拆方对Lewis肺癌荷瘤小鼠血中、脾中及瘤体中的树突状细胞(DC)含量的影响,以期阐明不同治则方药对机体免疫调节功能可能的作用机制。方法观察肺瘤平膏及其各拆方对Lewis肺癌小鼠血中及脾中DC含量的影响,并用免疫组化观察各组瘤组织中S-100蛋白的表达情况。结果 Lewis肺癌荷瘤小鼠的外周血及脾中DC含量(‰)较正常组明显降低(P<0.01),分别为:0.43±0.26vs4.68±0.90,0.32±0.16vs3.68±1.58,瘤组织中S-100蛋白的表达较弱;肺瘤平膏可明显提高其外周血及脾中DC含量(P<0.01),分别为:2.55±0.29,2.70±0.63,并明显上调S-100蛋白表达水平;拆方研究表明,肺瘤平膏中的益气类方药对Lewis肺癌荷瘤小鼠DC含量及表达的升高作用最为显著。结论在扶正培本为主要治则的肺瘤平膏抗肿瘤作用明确,其作用机制可能是通过提高荷瘤小鼠DC含量及表达,增强机体的抗肿瘤免疫功能,进而发挥抑瘤作用。

Objective To explore the possible immuno-regulating mechanism of action of Chinese drugs in different combinations （assembled depending different therapeutic principles） through observing the effects of Feiliuping intment （FLP） and its disassembled prescriptions on dendritic cells （DC） in blood,spleen and tumor in mice with transplanted Lewis lung cancer （LLC）.Methods Percentages of DC in blood,spleen and tumor of mice with transplanted LLC treated by FLP and its disassembled prescriptions were estimated,and the S-100 protein expression in tumor tissue was detected by immunohistochemical method.Results The percentage of DC （‰） in tumor bearing mice was 0.43±0.26 in peripheral blood,and 0.32±0.16 in spleen,significantly lower than those in normal mice 4.68±0.90 and 3.68±1.58,P0.01）;and S-100 protein expression in tumor was weakened.After FLP treatment,the percentages of DC （‰） in tumor bearing mice were increased to 2.55±0.29 in peripheral blood and 2.70±0.63 in spleen （P0.01）,with the S-100 protein expression in tumor tissue up-regulated significantly （P0.01）.Study on different assembled prescriptions of FLP showed that the qi supplementing components of FLP displayed the optimal actions.Conclusion FLP,a Chinese herbal prescription made depending on Chinese medicine therapeutic principle of strengthening body resistance and consolidating constitution,has an obvious anti-tumor effect,to improve the immunological anti-tumor function of organism by promoting the amount and expression of DC might be the possible intrinsic mechanism.