有氧运动训练对高蛋氨酸饮食大鼠血浆组织纤溶酶原激活物/纤维蛋白溶酶原激活因子抑制物-1和前列腺素I2/血栓烷A2系统的影响

Effects of aerobic exercise on plasma tissue plasminogen activator/plasminogen activator inhibitor-1 and postaglandin I2/thromboxanes A2 in rats fed a high methionine diet

目的 探讨有氧运动训练对高蛋氨酸饮食大鼠血浆组织纤溶酶原激活物/纤维蛋白溶酶原激活因子抑制物-1（t-PA/PAI-1）及前列腺素I2/血栓烷A2（PGI2/TXA2）系统的影响.方法 将雄性Wistar大鼠随机分为对照组、高蛋氨酸组及运动干预组.对照组喂饲普通饲料,高蛋氨酸组及运动干预组大鼠喂饲含3%蛋氨酸的特制饲料,同时运动干预组大鼠每日进行90min无负重游泳运动,每周训练6d.于实验进行8周后采用高效液相色谱法测定大鼠血浆同型半胱氨酸（Hcy）含量,选用发色底物法测定t-PA及PAI-1含量,选用放射免疫非平衡法测定6-酮-前列腺素F1（6-keto-PGF1）及TXB2含量.结果 高蛋氨酸组大鼠血浆Hcy含量显著高于对照组,t-PA/PAI-1及6-keto-PGF1α/TXB2比值均较对照组显著降低（P〈0.05）;与高蛋氨酸组比较,运动干预组血浆Hcy含量明显降低（P〈0.05）,t-PA/PAI-1及6-keto-PGF1α/TXB2比值显著升高（P〈0.05）,且上述各项指标与对照组间差异均无统计学意义（P〉0.05）.结论 高蛋氨酸饮食可诱发大鼠高半胱氨酸血症,导致血浆t-PA/PAI-1和PGI2/TXA2系统失衡;适宜有氧运动训练可降低高蛋氨酸饮食大鼠血浆Hcy水平,纠正t-PA/PAI-1及PGI2/TXA2系统失衡,从而预防高半胱氨酸血症发生.

Objective To observe the effects of aerobic training on plasma tissue plasminogen activator/plasminogen activator inhibitor-1 and postaglandin I2/thromboxanes A2 in rats fed a high methionine diet. Methods Ninetysix male Wistar rats were randomly divided into a normal diet control group （CR）, a high methionine diet group （MR）and a high methionine diet plus 90 min aerobic training intervention group （T ＋ MR）. After 8 weeks of training, the plasma level of homocysteine （Hcy） 6-keto-PGF1α, TXB2, t-PA and PAI-1 were measured. Results Plasma Hcy in the MR group increased two-fold as compared with the CR group, whereas t-PA/PAI-1 and 6-keto-PGF1α/TXB2 of the MR group decreased significantly. Plasma Hcy in the T ＋ MR group significantly decreased, while plasma t-PA/PAI-1 and 6-ketoPGF1α/TXB2 increased significantly compared with the MR group. The above parameters were not different in the T ＋ MR group from those of the CR group. Conclusions Hyperhomocysteinemia could be induced by a high methionine diet.Proper training can decrease the plasma Hcy level of rats fed with a high methionine diet, and correcting the imbalance of t-PA/PAI-1 and 6-Keto-PGF1α/TXB2 could prevent the development of hyperhomocysteinemia.