苯并噻二唑处理提高采后沙糖橘对指状青霉菌的抗性

Postharvest BTH Treatment Induces Resistance of Mandrin (Citrus reticulata Blancdo‘Shiyueju’) Fruit to Penicillium digitatum

对沙糖橘(Citrus reticulata Blancdo‘Shiyueju’)果实进行苯并噻二唑(S-methyl benzo thiadiazole-7-carbothioate,BTH)渗透处理后接种指状青霉菌孢子,12h后即可明显诱导果皮中抗病相关蛋白CR-CT1和CR-BG3基因表达并在贮藏期间维持较高水平;不同浓度BTH处理果实的发病率和病斑面积均比对照低,其中以0.025g·L-1BTH处理的效果最好;BTH处理提高了果皮几丁质酶(CT)、β–1,3–葡聚糖酶(GUN)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)活性和H2O2含量,表明BTH诱导的沙糖橘果实抗病性与果实CR-CT1和CR-BG3基因的表达增强,CT、GUN、PAL、PPO和POD等相关酶活性和H2O2含量提高密切相关。

Induction of disease resistance is one of the important strategies of biological control on fruit postharvest decay. The mandarin（Citrus reticulata Blancdo‘Shiyueju’）fruit were inoculated with Penicillium digitatum after vacuum infiltration of Benzothiadiazole（BTH）. Results showed that the expression of CR-CT1 and CR-BG3 substantially increased 12 h after BTH treatment. The morbidity and lesion area of green mold in mandarins,and the in vivo disease incidences of the fruit were signicantly reduced by different concentrations of BTH. BTH treatment induced the activities of CT,GUN,PAL, PPO,POD,and evoked the increase of H2O2 content. The results suggested that strong expression of CR-CT1 and CR-BG3,enhanced activities of several defense enzymes and H2O2 content are all involved in the induction of disease resistance by BTH treatment in mandarin fruit.